I'm an Industrial Biotechnology student in the B.S. Biomanufacturing program at Solano Community College. I'm interested in how biology, biomanufacturing, and computation work together — from expressing a protein in a cell to looking at its structure on a screen.
Right now I'm learning bioinformatics and structural biology tools alongside my lab coursework.
- 🏭 Biomanufacturing — producing proteins and other products with living cells
- 🧬 Molecular biology & proteins — gene expression, protein work, protein–ligand analysis
- 💻 Bioinformatics & computational biology — sequence analysis, structure validation, docking
- 🤖 AI for biology — an area I'm starting to explore
- 🔬 Also interested in cancer research and regenerative medicine
┌─ cancer-crispr-targets ───────────────────────────────────┐
│ TCGA-LUAD → driver genes → allele-specific CRISPR guides │
│ Corrects raw mutation frequency for gene length (TTN │
│ falls 2nd→25th, KRAS rises 9th→1st), then designs guides │
│ that tell the KRAS G12 mutant allele from the normal one.│
└───────────────────────────────────────────────────────────┘
┌─ crispr-guide-design ─────────────────────────────────────┐
│ SpCas9 guide design and off-target analysis for TP53 │
│ 2,860 PAM sites → 223 filtered guides, each searched │
│ against 10.5M sites on chromosome 17. │
└───────────────────────────────────────────────────────────┘
┌─ variant-calling-pipeline ────────────────────────────────┐
│ Nextflow: FASTQ → QC → alignment → VCF │
│ Containerised DSL2 pipeline with a generated test set │
│ of 25 planted variants, so recall can actually be │
│ measured. Run: 25/25 found, 0 false calls. │
└───────────────────────────────────────────────────────────┘
┌─ Lab work ────────────────────────────────────────────────┐
│ pGLO / GFP expression · molecular biology │
│ Transformed E. coli with the pGLO plasmid, prepared GFP │
│ lysate, purified GFP, did concentration / buffer │
│ exchange, and checked fluorescence under UV light. │
│ │
│ VNIAS · bioinformatics & docking internship │
│ Sequence analysis, structure validation, docking prep. │
└───────────────────────────────────────────────────────────┘
→ cancer-crispr-targets · crispr-guide-design · variant-calling-pipeline · roadmap · VNIAS
🗂️ Bioinformatics Portfolio → all projects on one board: status, next steps and stack
The computational projects were built with AI assistance (Claude Code); each repository says so and lists its limitations.
| Area | Tools |
|---|---|
| Sequence & structure validation | BLAST · PROCHECK · ERRAT |
| Structure visualization | Discovery Studio · UCSF Chimera |
| Molecular docking (learning) | AutoDock Vina · PyRx |
| Cancer genomics (learning) | R · maftools · TCGA MC3 data |
| Sequence analysis (learning) | Python · Biopython · NCBI Entrez |
| Workflows (learning) | Nextflow · containerised tools |
| Wet lab | Bacterial transformation · protein purification · buffer exchange |
The banner cell cycles through GFP expression on its own; open dark.svg directly and hover over the cell to light it up fully.
