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JulioLeonIncio/README.md

Julio Leon

Molecular neuroscientist at the Institute for Neurodegenerative Diseases, University of California, San Francisco. I use single-nucleus and spatial transcriptomics to study how glia and neurons change in the aging and degenerating human brain.

Current work

  • Nasu-Hakola disease: what DAP12 (TYROBP) loss does to human microglia and the tissue around them — snRNA-seq and Visium of frontal cortex and hippocampus (code: NHD-frontal-hippocampus-snRNAseq-Visium).
  • Layer I astrocytes in schizophrenia (anterior cingulate cortex; SCZ_ACC_L1_Astrocytes).
  • APOE and synapses in human cortex.

Methods I work with: 10x Chromium snRNA-seq, Visium, Cell Ranger / Space Ranger, Seurat, Harmony, BANKSY, cell2location, MAST and pseudobulk DESeq2, fgsea, CellChat; R and Python.

Background: PhD work on CAGE transcriptomics; postdoctoral research at RIKEN (Yokohama) on the aging brain before moving to UCSF.

Links: ORCID 0000-0002-1113-4544 · Google Scholar

Analysis code for published work is released under the MIT licence with a Zenodo DOI per paper; raw and processed data go to the repositories named in each paper's Data availability statement.

Popular repositories Loading

  1. guacamole guacamole Public archive

    Archived: first repository (2016)

  2. testing testing Public archive

    Archived: RStudio–GitHub connection test (2020)

    R

  3. PCAtools PCAtools Public

    Forked from kevinblighe/PCAtools

    PCAtools: everything Principal Components Analysis

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  4. CAGE-CAGE-Cap-Analysis-of-Gene-Expression-data-analysis CAGE-CAGE-Cap-Analysis-of-Gene-Expression-data-analysis Public

    CAGE (Cap Analysis of Gene Expression) data analysis: promoter-level quantification and differential expression

  5. Tutorial-from-DEseq2-to-GSEA-in-R Tutorial-from-DEseq2-to-GSEA-in-R Public

    Tutorial: from DESeq2 differential expression to gene-set enrichment (fgsea/GSEA) in R

  6. BayesPrism.Danko-Lab BayesPrism.Danko-Lab Public

    HTML