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BRR-uni/README.md

Hi, I'm Braulio 👋

I'm a chemical engineer and MSc in Biochemical Sciences interested in the biology of aging, with a focus on partial reprogramming and epigenetic rejuvenation. I build open-source tools for image analysis, data analysis, and lab automation.

  • 🔬 Interests: partial reprogramming, senescence, autophagy, quantitative microscopy
  • 🧰 Tools: Python · R · ImageJ/FIJI · Arduino · Android · 3D printing
  • 🌱 Currently: teaching chemistry and looking for research opportunities in aging/reprogramming
  • 📫 Reach me: braulio.uni@outlook.com

Featured Projects

  • ImageJ-nuclei-counter — FIJI/ImageJ plugins to automate segmentation and counting of nuclei from DAPI-stained confocal z-stack images, with semi-automated and fully automated versions.
  • OptoGeneLaser — Android app + Arduino code to control light stimulation frequency (on/off duration, cycles, rest periods) for optogenetics experiments via Bluetooth.
  • ImageJ_z_stack_plugins — FIJI/ImageJ plugins to automate z-projection (max intensity) and channel merging/enhancement for confocal microscopy images (CZI format).
  • Plots-and-Statistical-tests — Python/Jupyter scripts to generate time-series plots, correlation jointplots, and run statistical tests (normality, variance, group differences) from Excel data.
  • SBB_Chamber — 3D-printable 4-well chamber insert to improve Sudan Black B immunostaining on tissue slices, reducing sample loss and antibody usage.
  • Aromatic_Cycle_Grouper — Python script to identify aromatic cycles in molecules, group adjacent rings, and export results for molecular docking workflows.
  • ORM-Timer — Android dual-timer app for manual scoring of Object Recognition Memory tests in mice, with press-and-hold operation and countdown features.

Open to PhD positions, collaborations, and research opportunities in partial reprogramming, aging, and epigenetic rejuvenation.

Pinned Loading

  1. ImageJ-nuclei-counter ImageJ-nuclei-counter Public

    This plugins automates the image processing necessary to segment and count nuceli from presumably alive cells in a DAPI staining confocal z-stack projection

    ImageJ Macro

  2. ImageJ_z_stack_plugins ImageJ_z_stack_plugins Public

    A series of ImageJ plugins to run z stack imaging analysis faster

    ImageJ Macro

  3. OptoGeneLaser OptoGeneLaser Public

    An Android App to control the frequency of light stimulation in optogenetics stimulation experiments

    C++

  4. Plots-and-Statistical-tests Plots-and-Statistical-tests Public

    Python scripts to make time series plots from an excel file

    Jupyter Notebook

  5. SBB_Chamber SBB_Chamber Public

    This is a 3D printable file for a modified 4 Well chamber which facilitates Sudan Black B assays in tissue slices.